相关实验视频
Updated: Jun 11, 2025
06:59
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
10.3K
特定的外体微RNA和胃肠癌细胞的作用:当前理论
Bandar Almutairy1, Mohammad S Alzahrani2, Dania S Waggas3
1Department of Pharmacology, College of Pharmacy, Shaqra University, Shaqra 11961, Saudi Arabia.
Experimental cell research
|October 9, 2024
概括
胃肠道 (GI) 恶性瘤涉及复杂的危险因素. 携带微RNA (miRNA) 的外体是瘤微环境中的癌症进展和细胞间通信的关键调节者.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 胃肠道 (GI) 恶性瘤包括由可修改和不可修改的危险因素驱动的各种疾病.
- 了解癌症病因对于开发有效的治疗策略和改善患者治疗结果至关重要.
- 外基因组调解细胞间通信,转移分子信号,包括微RNA (miRNA),以调节细胞功能.
研究的目的:
- 研究外体微RNAs (miRNAs) 在瘤微环境中的调解相互作用中的调节作用.
- 为了阐明癌症衍生的外体细胞如何影响树皮细胞和免疫细胞.
- 突出外体miRNAs在癌症进展和治疗耐药性的重要性.
主要方法:
- 在胃肠道恶性瘤中的外体组合和功能分析.
- 癌症衍生的外体内微RNA (miRNA) 含量的表征.
- 检查外体miRNA转移及其对受体细胞 (肌瘤和免疫细胞) 的影响.
主要成果:
- 来自癌症的外体提供各种分子,包括高度的microRNAs (miRNAs),进入瘤微环境.
- 外体miRNAs显著影响关键的癌症特征,如免疫逃避,转移和亡抵抗.
- 外体miRNAs在癌细胞与周围的树皮细胞和免疫细胞之间的交叉声中起到关键的调解作用,调节基因表达和信号通路.
结论:
- 外体miRNAs在调节肠道恶性瘤瘤微环境中的细胞间通信中起着关键作用.
- 这些miRNAs在促进癌症进展,免疫逃避和对亡的抵抗方面发挥着关键作用.
- 向外体miRNAs为治疗胃肠道癌症提供了一个有前途的治疗途径.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:36Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:38Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K