在Aureobasidium pullulans中设计副产品通路,以进行高度净化的多酸发酵,同时恢复l-酸
Bingqin Li1, Jinzhao He1, Kangjia Zuo1
1College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
Bioresource technology
|October 9, 2024
概括
改造的Aureobasidium pullulans菌株通过增强多酸 (PMA) 发酵和减少副产品,显著改善了生物基的l-酸 (l-MA) 生产. 这导致高纯度的l-MA提取,促进可持续的化学制造.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 生物化学工程 生物化学工程
背景情况:
- 通过Aureobasidium pullulans的多酸 (PMA) 发酵生物基生产l-酸 (l-MA) 是有希望的,但受到影响最终产品质量的副产品的挑战.
- 优化发酵过程对于高效和高纯度的l-MA回收至关重要.
研究的目的:
- 设计Aureobasidium pullulans菌株以提高PMA合成和减少副产品的形成.
- 通过代谢工程和工艺优化,提高l-酸 (l-MA) 的整体生产率和产量.
主要方法:
- 通过过度表达马酸脱酶基因 (ApMDH2) 和删除pullulan,黑色素和氨酸通路中的基因来工程化底盘菌株.
- 实施了不可逆转的转酶系统,将NADPH转化为NADH,防止细胞内积累和增强PMA合成.
- 利用料批发发酵来优化工艺和提高生产率.
主要成果:
- 获得了最高的PMA标位194.3±1.1g/L和l-MA产量0.89±0.01g/g.
- 发酵生产率提高到1.45 ± 0.06 g/L·h.
- 从发酵中成功提取了86.19%的l-MA,纯度为99.7%.
结论:
- Aureobasidium pullulans 的代谢工程,再加上一个优化的转酶系统,显著提高了 PMA 和 l-MA 的产量.
- 开发的菌株和工艺使高效,高纯度的基于生物的l-酸生产成为可能,提供了可持续的替代品.
- 这项研究表明了改善微生物生产有价值的有机酸的可行策略.
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