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来自捐赠者的无细胞DNA液体活检以确定未来的移植拒绝
Weiwei Wang1,2, Cuello Garcia Haider2, Yinfeng Wang2
1College of Biology and Food Engineering, Suzhou University, Suzhou, Anhui Province, China.
Kidney research and clinical practice
|October 9, 2024
概括
来自捐赠者的无细胞DNA (dd-cfDNA) 液体活检显示出检测移植排斥的前景. 较高的dd-cfDNA水平与ABMR和TCMR等排斥类型相关,为传统活检提供了一个非侵入性的替代方案.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 移植免疫学 移植免疫学
- 分子诊断学 分子诊断学
背景情况:
- 移植脏的拒绝对移植的生存构成重大威胁.
- 传统的活检是侵入性的,并带有风险.
- 作为一种非侵入性的生物标志物,来自捐赠者的无细胞DNA (dd-cfDNA) 正在出现.
研究的目的:
- 评估dd-cfDNA液体活检在检测移植排斥中的潜力.
- 为了将dd-cfDNA水平与特定的排斥类型和移植功能相关联.
主要方法:
- 在体液中的dd-cfDNA度的分析.
- 在被排斥患者和健康对照患者的dd-cfDNA水平的比较.
- 确定不同类型的排斥和eGFR下降的切断值.
主要成果:
- 与健康人 (0.44%) 相比,抗体中介排斥 (ABMR) (2.45%) 和T细胞中介排斥 (TCMR) (1.3%) 的患者的dd-cfDNA水平显著更高.
- 25%的eGFR下降与2.5%的dd-cfDNA水平相关.
- 较高的dd-cfDNA度表明由于亡和亡过程的潜在排斥.
结论:
- dd-cfDNA液体活检为监测移植排斥提供了一种安全的,非侵入性的方法.
- 特定的dd-cfDNA值可以表明ABMR,TCMR和移植功能下降的风险.
- 快速的dd-cfDNA测定对于紧急评估是有价值的,需要进一步研究.
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