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缺少CREB3L1会损害牙细胞分化和牙沉积,部分通过TMEM30B
Yuanyuan Li1, Yuxiu Lin1,2, Jinqiang Guo1
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
International journal of oral science
|October 9, 2024
概括
循环腺单酸响应元素结合蛋白3像1 (CREB3L1) 对于牙生成至关重要. 它的删除会损害牙细胞分化和蛋白质分泌,部分通过TMEM30B,影响根部发育.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 牙细胞合成牙细胞外基质蛋白质,对牙产生至关重要.
- 循环腺单酸响应元素结合蛋白3像1 (CREB3L1) 在差异化牙细胞中得到丰富.
研究的目的:
- 研究CREB3L1在牙生成和牙细胞分化中的作用.
- 确定CREB3L1的下游目标和监管机制.
主要方法:
- 在Wnt1+血统小鼠模型中删除CREB3L1.
- 在小鼠牙细胞 (mDPCs) 上进行高通量测序 (ATAC-seq) 和RNA测序的转化酶可访问染色质的测试.
- 在体内和体外分析牙细胞分化,蛋白质合成和分泌.
主要成果:
- 删除CREB3L1导致根延伸和牙沉积不足.
- 缺乏CREB3L1减少了牙细胞分化,细胞内和细胞外蛋白质水平.
- 确定了牙矩阵蛋白1 (Dmp1),牙亚硫蛋白 (Dspp) 和跨膜蛋白30B (Tmem30b) 是直接转录的目标.
- TMEM30B 除损害了 odontoblastic 分化和蛋白质分泌;其过度表达在 CREB3L1 缺乏细胞中部分挽救了细胞外蛋白质分泌.
结论:
- CREB3L1对于牙生成和质细胞分化至关重要.
- CREB3L1直接增强了Dmp1,DSpp和其他分化基因的转录.
- CREB3L1间接促进蛋白质分泌,部分通过TMEM30B,影响牙形成.
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