在感染原生体的巨型病毒中,超寄生虫四分系统的复杂的转录规则
Alexandra Bessenay1, Hugo Bisio1, Lucid Belmudes2
1Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale (IGS), Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479), IM2B, IOM, Marseille, Cedex 9, France.
Nature communications
|October 9, 2024
概括
巨型病毒和病毒在宿主细胞内通过转录相互作用. 病毒细胞暂时改变巨型病毒的基因表达,并上调转型病毒,揭示复杂的病毒网络.
科学领域:
- 病毒学 病毒学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 超寄生症发生在病毒中,病毒会感染巨型病毒.
- 巨型病毒,病毒细胞和转录病毒共享调节元素,表明转录相互作用.
- 转基因病毒是病毒颗粒中发现的环节性DNA分子.
研究的目的:
- 为了研究巨型病毒 (M. chilensis),病毒 (Z. vitis) 和Acanthamoeba castellanii中的转型病毒 (M. vitis transpoviron) 之间的转录相互作用.
- 解读每个病毒伴侣的转录程序及其在共感染期间对其他病毒的影响.
- 了解在复杂的病毒-宿主-病毒细胞-转基因系统中控制基因表达的调节网络.
主要方法:
- 在被M. chilensis,Z. vitis和/或M. vitis transpoviron感染的Acanthamoeba castellanii细胞的时间过程RNA测序.
- 在整个感染周期中分析宿主细胞和病毒基因表达特征.
- 对比转录组分析以确定差异性基因表达模式.
主要成果:
- 巨型病毒感染显著改变了宿主细胞转录组.
- 转维龙对宿主,巨型病毒或病毒菌的基因表达没有明显的影响.
- 病毒细胞暂时调节巨型病毒基因表达,而不会改变病毒颗粒蛋白质组成.
- 病毒诱导了转型病毒基因的过度表达,可能是通过间接上调巨型病毒转录因子.
结论:
- 病毒共感染建立了复杂的,转录性调节的网络.
- 病毒细胞在调节巨型病毒和相关的转基因病毒的基因表达方面发挥着重要作用.
- 这项研究强调了宿主细胞内的多伙伴病毒系统中基因调节的复杂性.
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