高度COVID-19康复性血输血的生物化学理由
Caro Verbrugghe1,2, Elise Wouters1, Rosalie Devloo1
1Transfusion Research Center, Belgian Red Cross-Flanders, Ghent, Belgium.
Scientific reports
|October 9, 2024
概括
在康复性血 (CCP) 中的高亲和抗体对于有效的COVID-19治疗至关重要. 在疾病过程中早期输血高抗体度和亲和度的CCP最大化病毒结合,为治疗选择提供生物化学基础.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- 康复性血 (CCP) 输血是一种潜在的COVID-19治疗方法.
- 了解抗体与病毒结合的动力学对于优化CCP疗效至关重要.
研究的目的:
- 在接受CCP的患者中,模拟捐赠者抗体与SARS-CoV-2病毒结合.
- 为了确定CCP中IgG和IgA抗体的明显亲和力 (Kd,app) 和度.
- 建立基于抗体特征和病毒载荷的有效CPT输血标准.
主要方法:
- 开发一种免疫吸收试验来测量明显亲和力 (Kd,app).
- 使用抗体度和估计的病毒载量建模抗体结合.
- 用参考抗体和临床数据验证试验和模型.
主要成果:
- 89%的CCP捐献显示了两种可解决的IgG亲缘关系,中位数Kd,app为0.8nM.
- 中位数IgG度为44.0nM,明显高于IgA.
- 如果Kd,app<1nM和度>150nM,双重CPC输血 (500毫升) 达到>80%的抗体结合.
- 在疫苗接种前的捐赠中,只有4%的捐赠符合这些标准.
结论:
- 高抗体亲和度和CCP中的度对于有效的病毒中和至关重要.
- 当CCP在低至中等病毒载量时在疾病过程的早期给予时,可能是最有效的.
- 这项研究为选择具有COVID-19治疗最佳抗体特征的CCP捐赠提供了生物化学理由.
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