在大视野无透镜全息显微镜中通过二光子3D打印进行定量相位成像验证
Emilia Wdowiak1, Mikołaj Rogalski2, Piotr Arcab2
1Institute of Micromechanics and Photonics, Warsaw University of Technology, 8 Sw. A. Boboli St., Warsaw, 02-525, Poland. emilia.wdowiak.dokt@pw.edu.pl.
Scientific reports
|October 9, 2024
概括
使用大面积3D打印和无透镜数字全息显微镜 (LDHM) 来评估定量相成像 (QPI) 的一致性. 这项研究对大型FOV无透镜相位成像进行了基准测试,这对于生物医学应用至关重要.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜的使用方法
- 光子学 是一个光子学.
背景情况:
- 具有高分辨率的大视野 (FOV) 显微镜对于无标签的生物医学成像至关重要.
- 无镜头数字全息显微镜 (LDHM) 是有前途的,但在大面积的精确定量相成像 (QPI) 中面临着挑战.
- 现有的用于测试QPI系统的幻影仅限于小型FOV,并且不能完全解决无镜头技术的扭曲.
研究的目的:
- 提出和验证一种方法来评估大FOV无透镜数字全息显微镜 (LDHM) 的相一致性.
- 适应大面积的两光子聚合 (TPP) 用于制造适合无镜头成像的相位测试目标.
- 在LDHM系统的整个FOV中量化相位成像误差.
主要方法:
- 使用大面积二光子聚合 (TPP) 与加尔沃和皮埃佐扫描制造广场相位测试目标的制造.
- 在LDHM中在单拍和多的代双图像最小化条件下对目标的审查.
- 使用林尼克干扰计对LDHM结果进行交叉验证.
主要成果:
- 通过使用TPP.成功制造了大面积阶段测试目标.
- 在整个FOV中验证了LDHM相位成像错误,区域之间的相位值差异低于12%.
- 证明了TPP与LDHM结合用于评估相位一致性的有效性.
结论:
- 双光子聚合 (TPP) 是一种可行的技术,用于创建无镜头成像的大面积相位测试目标.
- 当与TPP制造的目标进行评估时,LDHM相位成像在大型FOV中表现一致.
- 对于精确的大面积光子制造,需要考虑新的设计,以提高LDHM的精度.
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