在Mycobacterium的双空间宿主-细菌基因表达呼吸道感染
Federico Di Marco1,2,3, Francesca Nicola1,4, Francesca Giannese3
1Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious, IRCCS Ospedale San Raffaele, Milan, Italy.
Communications biology
|October 9, 2024
概括
这项研究表明,定制的细菌探针可以使用空间转录学绘制FFPE组织中的宿主-病原体相互作用图. 这种技术将细菌基因表达与肺部感染中的宿主反应相关联,推进了微生物病原性研究.
科学领域:
- 微生物学 微生物学
- 病变的发生和发病.
- 分子生物学分子生物学
背景情况:
- 了解宿主-病原体相互作用对于微生物病原体产生至关重要.
- 空间转录学提供了一个强大的工具来研究组织微环境中的这些相互作用.
- 甲固定嵌 (FFPE) 组织广泛用于回顾性研究.
研究的目的:
- 为了证明定制的细菌探针在FFPE组织中的空间转录组学的实用性.
- 在老鼠肺部慢性Mycobacterium疹感染期间识别宿主-病原体相互作用.
- 为了将细菌基因表达与宿主反应和组织状况相关联.
主要方法:
- 开发定制的细菌探针,针对Mycobacterium基因 (rpoB和lsr2).
- 在FFPE小鼠肺组织上应用基于探针的空间转录学.
- 分析共定位的细菌和宿主转录基因数据.
- 评估与细菌基因表达有关的氧化应激标志物.
主要成果:
- 在FFPE组织中成功检测了与宿主基因表达一起的细菌转录.
- 观察到rpoB表达,气道中的细菌负载和lsr2毒性因子表达之间的相关性.
- 增加的lsr2表达与高氧化应激的肺组织有关.
- 证明了双重细菌和宿主基因表达分析的可行性.
结论:
- 定制的细菌探针可以在FFPE组织中进行空间转录学分析.
- 这种方法可以同时检测宿主和细菌的转录组.
- 这些发现为在临床样本中对微生物病原发生的先进研究铺平了道路.
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