Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.1K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.1K
Sanger Sequencing01:57

Sanger Sequencing

753.7K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
753.7K
DNA Microarrays02:34

DNA Microarrays

17.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K
Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Next-generation Sequencing03:00

Next-generation Sequencing

88.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.3K
DNA as a Genetic Template02:05

DNA as a Genetic Template

6.8K
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

DNA methylation profiles at hospital admission are associated with subsequent severe COVID-19 outcomes.

Clinical epigenetics·2026
Same author

Library Preparation for Genome-Wide DNA Methylation Profiling.

Bio-protocol·2025
Same author

Circulating endothelial signatures correlate with worse outcomes in COVID-19, respiratory failure and ARDS.

Critical care (London, England)·2025
Same author

Defining the cell and molecular origins of the primate ovarian reserve.

Nature communications·2025
Same author

Testicular somatic and germ cell maturation during rhesus macaque development.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

DNA methylation predicts infection risk in kidney transplant recipients.

Life science alliance·2025
Same journal

OpenIMC: an open-source platform for analyzing single-cell and spatial proteomics by imaging mass cytometry.

BMC bioinformatics·2026
Same journal

NAP: an open source pipeline for cross-domain microbiome profiling using Nanopore sequencing-derived amplicon data.

BMC bioinformatics·2026
Same journal

SurvGME: an R package for survival analysis with graphical and measurement error models.

BMC bioinformatics·2026
Same journal

SimMapNet: a Bayesian framework for gene regulatory network inference using gene ontology similarities as external hint.

BMC bioinformatics·2026
Same journal

Dual channel drug-drug interactions extraction based on cross attention.

BMC bioinformatics·2026
Same journal

FeSseqdb: a curated sequence-level database and interpretable machine learning framework for identifying iron-sulfur proteins.

BMC bioinformatics·2026
查看所有相关文章

相关实验视频

Updated: Jun 11, 2025

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.8K

甲基SeqLogo:DNA甲基化智能序列标志

Fei-Man Hsu1, Paul Horton2

  • 1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, USA.

BMC bioinformatics
|October 9, 2024
PubMed
概括
此摘要是机器生成的。

MethylSeqLogo可视化转录因子结合部位的DNA甲基化模式,揭示序列特定的甲基化偏好. 这个工具有助于理解DNA甲基化如何影响转录因子结合特异性.

关键词:
通过DNA甲基化.转录因子绑定站点视觉化的可视化

更多相关视频

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.0K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.6K

相关实验视频

Last Updated: Jun 11, 2025

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.8K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.0K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.6K

科学领域:

  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 转录因子可以与甲基化DNA结合,从而可能增加结合特异性.
  • DNA甲基化模式是特定于组织的,并提供额外的监管信息.
  • 双硫酸盐测序提供了研究DNA甲基化现象的数据.

研究的目的:

  • 开发一种用于可视化DNA甲基化在转录因子结合部位的新方法.
  • 扩展序列标志表示,包括甲基化数据.
  • 分析与DNA甲基化有关的转录因子结合偏好.

主要方法:

  • 开发了MethylSeqLogo,这是序列标志的扩展,包含DNA甲基化和代表性不足的二元信息.
  • 考虑的DNA序列上下文 (CpG等) 和基因组区域 (促进者与整个基因组).
  • 显示来自DNA链和保存序列标志语义的信息.

主要成果:

  • 展示了MethylSeqLogo与CpG结合转录因子的实用性.
  • 鉴定了像MYC和ZBTB33这样的转录因子,这些转录因子可以结合非甲基化CpG位点.
  • 观察到甲基化中性结合对于诸如CEBPB等因素.

结论:

  • 通过MethylSeqLogo,可以探索二硫酸盐和ChIP测序数据.
  • 该软件生成出版质量的数据,用于分析DNA甲基化和转录因子结合.