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测序平台对使用Hawk-SeqTM检测化学突变的灵敏度的影响

Sayaka Hosoi¹, Takako Hirose¹, Shoji Matsumura²

¹R&D - Safety Science Research, Kao Corporation, 3-25-14 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-0821, Japan.

Genes and environment : the official journal of the Japanese Environmental Mutagen Society

|October 10, 2024

概括

使用Hawk-SeqTM的错误纠正下一代测序 (ecNGS) 可以在不同的测序平台上可靠地检测突变性. 虽然背景突变频率因平台而异,但都准确地反映了变异原体的特征.

科学领域:

基因组学就是基因组学.
毒理学毒理学毒理学
生物技术是生物技术.

背景情况:

经过错误纠正的下一代测序 (ecNGS) 能够在突变原体暴露后进行全基因组突变分析.
霍克-SeqTM是一种已建立的ecNGS方法,用于突变致病性评估.
技术可转移性和对ecNGS敏感性的平台特定影响需要评估.

研究的目的:

评估Hawk-SeqTM协议在各种测序平台上的技术可转移性.
评估不同测序仪器对突变数据质量和突变性评估的影响.
为了确定测序平台的差异是否会影响ecNGS分析的灵敏度和可靠性.

主要方法:

通过使用Hawk-SeqTM协议评估了四个测序平台 (HiSeq2500,NovaSeq6000,NextSeq2000,DNBSEQ-G400).
使用的小鼠骨髓DNA样本暴露于[a]烯 (BP) 作为变异原体.
分析了整体突变 (OM) 频率和三核酸突变模式.

主要成果:

所有四个平台都检测到BP暴露后G:C到T:A突变的剂量依赖性增加.
总体突变频率因平台而异,NextSeq2000显示较高的背景G:C到C:G突变.
突变模式的高共因相似性 (0.92-0.95) 表明了平台等效突变原体的特征反射.

结论:

在所有评估平台上,Hawk-SeqTM分析有效地检测出了突变原体诱导的突变.
背景错误类型和频率的平台特定差异被观察到.
在进行ecNGS突变性实验之前,评估测序平台效应至关重要.

关键词:

错误纠正的序列编排是错误纠正的突变性测定试验突变性测定试验突变的签名突变的签名下一代测序测序是什么

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