通过经过验证并经过BAGI评估的UPLC-MS/MS方法在血中确定极度溶解物ectoine,并对大鼠进行药理动力学研究
Mahmoud Rabee1, Ragab A M Said2,3, Ibrahim A Naguib4
1Research and Development Department, Heliopolis University, Cairo, 11785, Egypt. Mahmoud.rabee@hu.edu.eg.
BMC chemistry
|October 10, 2024
概括
这项研究开发了一种敏感的UPLC-MS/MS方法,用于量化大鼠血中的ECT. 经过验证的方法准确测量了ECT水平,并用于研究其口服后的药理动力学行为.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 爱托因 (ECT) 显示出对各种人类疾病的治疗潜力.
- 在生物矩阵中精确量化ECT对于理解其影响至关重要.
- 开发一种可靠的ECT血水平测定方法至关重要.
研究的目的:
- 开发和验证一种敏感的超高性能液体染色学-并联质谱法 (UPLC-MS/MS) 方法,用于量化大鼠血中的ECT.
- 为了研究口服后 ECT 在大鼠中的药理动力学概况.
主要方法:
- 从大鼠血中提取ectoine,使用蛋白质沉和基ectoine作为内部标准.
- 在UPLC C8柱上进行染色学分离,其中具有酸和酸的移动相.
- 使用多重反应监测 (MRM) 在正离子模式下通过电子喷射电离质谱法 (ESI-MS) 检测.
主要成果:
- 通过UPLC-MS/MS方法,在1-1000 ng/mL范围内显示出线性,恢复率高 (96.48-97.37%).
- 该方法根据FDA的指导方针进行了验证,显示出良好的准确性,精度,并且没有显著的矩阵效应.
- 经过验证的方法已成功应用于定量ECT在刺鼠血中,并分析其药理动力学.
结论:
- 已经建立了一种敏感且经过验证的UPLC-MS/MS方法,用于在老鼠血中量化ectoine.
- 这种方法适用于对ectoine的药理动力学研究.
- 这项研究为进一步研究ectoine的治疗应用提供了基础.
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