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从人类肠道中获得的GH29 fucosidases的生物信息学辅助功能探索Parabacteroides

Haiyang Wu¹, Qingxin Li¹, Jin Chuan Wu¹

¹Guangdong Engineering Technology Research Centre of Enzyme and Biocatalysis, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, No. 10 Shiliugang Road, Haizhu District, Guangzhou, Guangdong 510316, P. R. China.

|October 10, 2024

概括

肠道细菌拥有的α-L-fucosidases对于分解复杂糖至关重要. 这项研究分析了Parabacteroides中的GH29 fucosidases,揭示了氨酸O-甘氨酸降解和宿主健康所必需的各种特异性.

科学领域:

微生物学微生物学
葡萄糖生物学葡萄糖生物学
酶学是一种酶学.

背景情况:

肠道微生物,特别是Parabacteroides,在宿主健康中发挥作用,但它们的碳水化合物活性酶 (CAZymes) 尚未得到充分研究.
阿尔法-L-fucosidases是肠道微生物生产的关键酶,用于糖利用.

研究的目的:

为了分析肠道Parabacteroides的CAZome,专注于GH29的fucosidases.
预测和功能验证新型GH29fucosidases的基质特异性.

主要方法:

对11种Parabacteroides类型菌株的CAZome分析.
对于46个GH29的功能概况的in silico预测. fucosidases.
对四个新型GH29序列的酶性验证.

主要成果:

准细菌菌类有能力降解粘素O-甘氨酸,其中丰富的GH29是fucosidases.
超过一半的预测GH29酶作为alpha1,3/4 fucosidases起作用,这对于易斯抗原表位很重要.
四种新型GH29酶表现出各种特异性,包括严格的alpha1,3/4 fucosidase活性和广泛的alpha1,2/3/4/6链接识别.

结论:

在Parabacteroides中的特定GH29 fucosidases,如PgoldGH29B和PgoldGH29D,有助于粘素O-甘氨酸的降解.
了解这些酶可以了解Parabacteroides在肠道微生物群中的生态作用.
在其他物种中进一步探索微生物CAZymes是有必要的.

关键词:

这就是为什么CUPPPP CUPP.GH29 贝尔特 (BERT) 是一个人.肠道上的巴巴克特里奥伊德.基质特异性基质特异性 α-l-fucosidase 的使用情况.

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