解热化SERS生物传感器,使其能够转化为易于使用的测试方法
Lutfun Naher1, Steven M Quarin1, Der Vang1
1Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA. strobbpo@ucmail.uc.edu.
Analytical methods : advancing methods and applications
|October 10, 2024
概括
冷干燥 (软化) 提高了无试剂表面增强拉曼散射 (SERS) 传感器的稳定性和保质期,用于照顾点检测病原体. 这提高了SERS传感器用于远程诊断的可用性.
科学领域:
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 治疗点 (POC) 病原体检测对于疾病控制至关重要.
- 无反应剂表面增强拉曼散射 (SERS) 传感器提供快速的病毒识别.
- 目前的SERS测试在管理,运输和储存方面面临挑战.
研究的目的:
- 为了提高无试剂SERS传感器的稳定性和保质期,使用冷化.
- 为了评估冷保护剂度对传感器性能的影响.
- 评估冷化SERS传感器用于检测SARS-CoV-2变种的实用性.
主要方法:
- 无反应剂的SERS传感器经过冷干燥 (冷化) 过程.
- 为了优化稳定性,研究了各种度的冷保护剂.
- 使用混合和检测方法测试了冷化传感器,用唾液样本添加了SARS-CoV-2DNA寡核酸.
- 研究了软化对传感器内的DNA催化机制的好处.
主要成果:
- 软化显著提高了无试剂SERS传感器的稳定性和保质期.
- 使用冷化传感器的混合检测方法证明了有效检测SARS-CoV-2变种.
- 发现冷化有利于使用DNA催化机制的传感器.
结论:
- 解热化是一种可行的方法,可以提高无试剂SERS传感器的实用性和可用性.
- 这种技术有助于将SERS技术用于POC测试在资源有限和远程环境中的翻译.
- 改进的传感器稳定性支持开发可访问的全球健康诊断工具.
更多相关视频
08:26Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
12.0K
09:52An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
Published on: September 15, 2020
3.1K
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
14.1K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
14.1K
01:17Microbial Biosensors
88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88