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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 11, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

通过独特的分子标识符和高通量测序简化RNA Aptamer选择.

Dengwei Zhang^1,2, Yuqing Liu^1,2, Huidong Huang¹

¹The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.

Analytical chemistry

|October 10, 2024

概括

我们开发了ID-SELEX,这是一种使用独特分子标识符的高效RNA吸收酶选择方法. 这种方法加速了细胞成像,药物输送和治疗方面的应用.

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

相关实验视频

Last Updated: Jun 11, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

科学领域:

生物技术是生物技术.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

由于它们的结构多样性,更高的亲和力和特异性,RNA吸收体在细胞成像和治疗等应用中比DNA吸收体具有优势.
传统的RNA胺体选择方法是漫长的,需要多轮选,这阻碍了它们的广泛应用.

研究的目的:

引入一种高效的截断选择方法,ID-SELEX,用于快速发现RNA吸收酶.
为了克服传统的aptamer选择过程的耗时性质.

主要方法:

开发了ID-SELEX (识别DNA-SELEX),将独特的分子标识符 (UMI) 纳入每个模板.
利用高通量测序来分析UMI标记的阿巴候选物,减轻PCR放大偏差.
应用ID-SELEX来选择针对人类结肠癌细胞 (HCT-8) 和小鼠肌细胞细胞 (C2C12) 的向细胞.

主要成果:

仅在两个选择轮中,成功地确定了针对HCT-8细胞的高质量亚胺.
在单个选择轮中,选择了六个向C2C12细胞的RNA受体.
证明了ID-SELEX在不同细胞系和物种中的效率和多功能性.

结论:

ID-SELEX 能够使用高通量测序和 UMI 标记进行快速的 RNA 适合体查.
这种方法促进了人类和小鼠细胞系的快速体发现.
ID-SELEX提供了一种具有生物医学研究,临床应用和精密医学潜力的新型分子工具.