使用全基因组关联研究剖析向日圆盘直径的遗传结构
Yavuz Delen1, Ravi V Mural2, Semra Palali-Delen1,3
1Department of Agronomy and Horticulture University of Nebraska-Lincoln Lincoln NE USA.
Plant direct
|October 10, 2024
概括
太阳花圆盘直径对于石油产量至关重要,具有很高的遗传性 (0.88). 全基因组关联研究确定了重要的遗传位置和候选基因,为改善日油生产铺平了道路.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 提高作物质量 提高作物质量
背景情况:
- 太阳花 (Helianthus annuus L.) 是全球重要的食用油来源.
- 太阳花的种子产量受各种成分特征的影响,包括圆盘直径.
- 了解磁盘直径的遗传基础是提高向日油产量的关键.
研究的目的:
- 为了评估多年来向日圆盘直径的表型变化.
- 为了估计磁盘直径特征的遗传性.
- 通过全基因组关联研究 (GWAS) 识别与日圆盘直径相关的遗传位置和候选基因.
主要方法:
- 在三年内 (2019年,2020年,2022年) 从342个向日加入中收集磁盘直径的表型数据.
- 使用可调节的基因型测序 (tGBS) 来对274个加入进行基因型化,生成226,779个单核酸多态 (SNP).
- 全基因组关联研究 (GWAS) 采用混合线性模型 (MLM) 和固定和随机模型循环概率统一 (farmCPU) 方法.
主要成果:
- 盘直径的广义遗传性 (H2) 估计为0.88.
- GWAS确定了106个与磁盘直径相关的显著SNP (MLM) 和8个与磁盘直径相关的显著SNP (farmCPU).
- 在染色体10和16上检测到重要的信号,涉及到特定的遗传位置和候选基因.
结论:
- 太阳花圆盘直径是一种高度遗传的特征.
- 鉴定出基因位置和候选基因为标记器辅助选择和育种策略提供了基础.
- 这项研究提供了通过遗传增强磁盘直径来提高日油产量的潜力.
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