PpBBX32和PpZAT5调节桃子果中的温度依赖和组织特异的安托西亚宁积累
Dan Huang1, Lei Xue1, Yueqin Lu2
1Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University Zijingang Campus, Hangzhou, PR China.
Horticulture research
|October 10, 2024
概括
研究人员确定了两种关键基因,即PpBBX32和PpZAT5,它们调节桃子中素 (果色素) 积累. 这些基因激活PpMYB10.1,根据温度和组织类型影响颜色,而斯蒙酸盐起着作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 果实科学 果实科学
- 生物化学 生物化学
背景情况:
- 氨酸对水果的质量和营养至关重要.
- 转录因子PpMYB10.1对于桃子中安东的积累至关重要.
- PpMYB10.1的上游调节者控制可变的素积累仍然不清楚.
研究的目的:
- 确定PpMYB10.1.1.的上游监管因素.
- 研究PpBBX32和PpZAT5在素积累中的作用.
- 阐明温度依赖和组织特异性酸盐积累的机制.
主要方法:
- RNA-Seq分析以确定候选基因.
- 暂时表达和基因转变用于功能特征.
- 相互作用测试 (促进体激活和蛋白质与蛋白质相互作用).
- 在分析和甲基斯蒙酸盐 (MeJA) 处理.
主要成果:
- 鉴定出PpBBX32和PpZAT5是对抗生素积累的积极调节者.
- 这两种蛋白质都直接激活PpMYB10.1促进体,形成一个复合体.
- 安索亚宁积累,PpBBX32和PpZAT5的表达与斯酸盐水平相关.
- 治疗MeJA促进了安东素的积累.
结论:
- PpBBX32和PpZAT5作为PpMYB10.1.1.的上游激活剂.
- 贾斯莫纳酸盐参与通过PpBBX32和PpZAT5.5调节氨酸的积累.
- 这项研究揭示了桃子中差异性抗生素积累的关键机制.
更多相关视频
08:48Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
Published on: December 16, 2016
10.6K
08:08Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
Published on: June 16, 2020
7.2K
相关概念视频
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
Biological Clocks and Seasonal Responses
34.6K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.6K
Epistasis
46.3K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
46.3K
