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高度稳定的Saccharomyces cerevisiaeL-BC体具有多功能包装潜力

Enrika Celitan¹, Ramunė Stanevičienė², Elena Servienė²

¹Laboratory of Nucleic Acid Biochemistry, Department of Biochemistry and Molecular Biology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.

Frontiers in bioengineering and biotechnology

|October 10, 2024

概括

病毒样颗粒 (VLP) 在纳米输送应用中表现出极好的稳定性. 酵母衍生的VLP在较高的温度下表现出优越的稳定性,与细菌衍生的VLP相比,突出了它们的纳米传递潜力.

科学领域:

纳米生物技术纳米生物技术
疫苗开发疫苗开发
药物输送系统药物输送系统

背景情况:

病毒样粒子 (VLP) 是先进的纳米,在疫苗和纳米输送中具有广泛的应用.
对于它们在纳米生物技术中的成功应用,VLP完整性至关重要.
VLP的生产和功能化是研究的关键领域.

研究的目的:

为了描述Saccharomyces cerevisiae L-BC VLP的稳定性.
为了比较细菌衍生的与酵母衍生的L-BC VLP的稳定性.
评估L-BC VLP的封装能力,以实现功能化.

主要方法:

从大肠杆菌和麦芽菌中合成和净化L-BC VLP.
在不同的离子强度,pH值和温度条件下测试VLP的稳定性.
通过基因工程封装红色光蛋白mCherry,通过被动扩散封装尼.

主要成果:

在各种离子强度,pH值和离子度 (高达37°C) 中,L-BC VLPs表现出显著的尺寸稳定性.
与细菌衍生的VLP相比,酵母衍生的VLP在酸性pH下表现出更高的稳定性,高离子强度和升高的温度.
成功地将mCherry和nisin封装到L-BC VLPs中,展示了功能化潜力.

结论:

L-BC VLP具有很高的长期稳定性,使其适合纳米输送系统.
酵母衍生的L-BC VLP比细菌衍生的同类产品具有更高的热稳定性.
这些发现强调了L-BC VLPs作为多功能纳米交付平台的潜力.

关键词:

在L-BC病毒中.这种植物是Saccharomyces cerevisiae.封装的封装方式粒子稳定性粒子稳定性类似病毒的颗粒.

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