相关实验视频

Updated: Jun 11, 2025

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

10.8K

加快的依赖于融合的内体体逃逸改善了递送的酶载荷的活性

Angel Luis Vázquez Maldonado, Teresia Chen, Diego Rodriguez

    bioRxiv : the preprint server for biology
    |October 10, 2024
    PubMed
    概括

    研究人员开发了AV5.3,这是一种微型蛋白质,可以提高生物物质的进入细胞的速度. 这种新的蛋白质能够更早地进行内体逃逸,改善像二叶酸还原酶 (DHFR) 这样的治疗酶的活性.

    更多相关视频

    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay09:19

    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

    Published on: October 19, 2012

    14.0K
    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy08:55

    Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

    Published on: December 29, 2017

    9.5K

    相关概念视频

    SNAREs and Membrane Fusion01:43

    SNAREs and Membrane Fusion

    Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
    SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
    10.8K
    Maturation of Endosomes01:28

    Maturation of Endosomes

    The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
    Changes in location
    The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
    4.2K
    Recycling Endosomes and Transcytosis00:58

    Recycling Endosomes and Transcytosis

    The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
    The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
    2.6K
    Intralumenal Vesicles and Multivesicular Bodies01:38

    Intralumenal Vesicles and Multivesicular Bodies

    Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
    3.4K
    Fusion of Secretory Vesicles with the Plasma Membrane01:26

    Fusion of Secretory Vesicles with the Plasma Membrane

    Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
    In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
    11.0K
    Rab Cascades01:25

    Rab Cascades

    Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
    2.6K