相关实验视频

Updated: Jun 11, 2025

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

在疹病毒核出口期间,CLCC1促进了膜融合

Bing Dai^1,2, Lucas Polack¹, Adrian Sperl^1,2

¹Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America.

bioRxiv : the preprint server for biology

|October 10, 2024

概括

疹病毒劫持了一个涉及CLCC1的细胞膜融合机制,用于核输出. 这一发现揭示了核外形成和病毒囊体运输所必不可少的古老过程.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

疹类 (Herpesvirales) 感染了包括人类在内的各种物种.
疹病毒通过涉及核膜芽和融合的非正规途径将囊从核转移到细胞质.
核出炉核聚变阶段的分子媒介在很大程度上是未知的.

研究的目的:

为了确定宿主因子,对于核出口融合阶段的简单疹病毒1感染必不可少.
阐明已识别的宿主因子在病毒囊运输和核膜形态发生过程中的作用.

主要方法:

使用简单疹病毒1进行全基因组CRISPR选.
进行了功能性测试,以评估宿主因子枯竭对病毒核退出和细胞过程的影响.

主要成果:

鉴定出CLCC1是疹病毒核出口的融合阶段的关键宿主因素.
失去CLCC1导致核出口的缺陷,围核囊泡的积累,并减少病毒标位.
此外,CLCC1还参与了核毛孔复合体在未感染细胞中的插入.
在感染软体动物和鱼类的古老疹病毒中保存了CLCC1的同类物.

结论:

在疹病毒核退出过程中,CLCC1是一个重要的宿主因子,它调解了周核病毒与外核膜的融合.
这项研究揭示了一种古老的膜融合机制,对核外形态发生至关重要,疹病毒利用这种机制来有效地运输囊.
这些发现表明,CLCC1在病毒感染和基本细胞过程中都起着保留作用.

关键词:

在 CLCC1 中,这是一个CRISPR屏幕.在HSV-1病毒中,化物道化物道疹简单病毒病毒.疹病毒疹病毒膜融合融合是什么膜融合是什么核能正在芽中的一个地方.核出口核出口核出口核孔插入的核孔插入.核细胞质体运输是核细胞质体运输.

更多相关视频

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Published on: August 16, 2013

相关概念视频

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

SNAREs and Membrane Fusion

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...

Clathrin Coated Vesicles

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

Fusion of Secretory Vesicles with the Plasma Membrane

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...

Pinching-off of Coated Vesicles

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...