相关实验视频
Updated: Jun 24, 2026

10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
36.9K
鼻腔微生物以不同的方式在人类鼻腔表皮器官中定居并引起细胞因子
Andrea I Boyd1,2, Leah A Kafer1,2, Isabel F Escapa1
1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
bioRxiv : the preprint server for biology
|October 10, 2024
概括
黄金葡萄球菌或肺炎链球菌的鼻子殖民可能会增加感染风险,而Dolosigranulum物种表明健康. 人类鼻腔器官模型这些宿主-微生物相互作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 宿主-微生物相互作用
背景情况:
- 黄金葡萄球菌 (Staphylococcus aureus) 或肺炎链球菌 (Streptococcus pneumoniae) 的鼻腔殖民与更高的感染风险有关.
- 相反,Dolosigranulum物种的鼻腔殖民与健康状态有关.
研究的目的:
- 在人类鼻腔粘膜中研究宿主-微生物动态,使用一种新的有机体模型.
- 为了比较人类鼻腔有机体 (HNO) 与黄金葡萄球菌,肺炎菌和猪菌菌 (Dolosigranulum pigrum) 的殖民作用.
主要方法:
- 人类鼻上皮质器官 (HNO) 被S. aureus,S. pneumoniae或D. pigrum单独殖民长达48小时.
- 分析了细菌局部化,细胞毒性和宿主细胞因子反应.
- 测量了化学激素的表达 (CXCL10,CXCL11).
主要成果:
- HNO成功支持单一殖民,细菌局部化到粘液层和最小的细胞毒性.
- 鼻上皮表现出特定物种和一般的细胞因子反应,但不是I型干扰素,表明殖民而不是感染.
- 活生生的金黄色细菌诱导IL-1家族细胞因子,表明炎症酶激活.
- 在D. pigrum和活的S. aureus下降了CXCL10,而S. pneumoniae增加了CXCL11.
结论:
- 人类鼻上皮质有机体作为一个有价值的模型,用于研究鼻膜中的宿主-微生物动态.
- 这项研究揭示了不同的宿主对不同细菌物种的殖民反应的不同,包括炎症信号和化学激素调制.
相关概念视频
Introduction to the Human Microbiota
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
Microbiome of the Eye
The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Microbiota of the Respiratory Tract
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...
Atypical Pneumonia
Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...

