从工程生物材料中控制释放微生物
bioRxiv : the preprint server for biology
|October 10, 2024
概括
工程生物材料通过一种新的水凝骨折机制释放益生菌. 这种方法确保了持续的,可控的活性益生菌的治疗应用.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 药物运输 药物运输 药物运输
背景情况:
- 益生菌调节微生物组和免疫反应,为复杂疾病提供治疗效益.
- 有效的益生菌治疗需要持续的持久性和在目标部位的殖民化.
- 现有的方法缺乏生物材料来持续释放代谢活性益生菌.
研究的目的:
- 开发一种用于持续释放活性益生菌的新生物材料.
- 展示一种控制益生菌输送的通用机制.
- 为了克服反复给予益生菌的局限性.
主要方法:
- 在不那么硬的水凝中封装硬的益生菌微生物.
- 利用微生物增殖诱导水凝骨折和释放.
- 通过矩阵属性来描述释放动力学和控制输送.
主要成果:
- 工程生物材料 (ELM) 实现益生菌的零顺序释放动力学.
- 小型ELM在2小时内释放大于10^8个殖民地形成单位 (CFU) 的大肠杆菌,持续超过10天.
- 益生菌的释放可以通过初始细胞负载和矩阵机械性质来控制.
结论:
- 通过水凝骨折证明了一种通过水凝骨折持续释放益生菌的新型通用机制.
- 这种方法可以控制各种益生菌类型 (细菌和真菌) 的输送.
- 这项技术有可能用于治疗需要益生菌治疗的广泛疾病.
相关概念视频
Biological Methods for Microbial Control
2
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
2
Microorganisms in Medicine and Therapeutics
2
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
2
Environmental Applications of Microorganisms
3
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
3
Types of Microorganisms
3
Microorganisms are a diverse group of microscopic entities broadly categorized into cellular and acellular types based on their structural organization. Cellular microorganisms include bacteria, archaea, fungi, protozoa, and algae, while acellular microorganisms are represented by viruses.Cellular MicroorganismsBacteriaBacteria, tiny prokaryotic organisms, exhibit fascinating shapes such as rods, spheres, and spirals. They adapt to diverse habitats, including soil, water, and human-associated...
3
Methods for Controlling Microbial Growth
3
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
3
Microorganisms in Agriculture and Food industry
3
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
3


