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相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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相关实验视频

Updated: Jun 11, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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埃拉:在高分辨率的空间转录学中模拟细胞内的基因表达的亚细胞空间变异.

Jade Xiaoqing Wang, Xiang Zhou

    bioRxiv : the preprint server for biology
    |October 10, 2024
    PubMed
    概括

    我们开发了亚细胞表达本地化分析 (ELLA),这是一个计算工具,可以使用高分辨率空间转录学来模拟细胞内的mRNA本地化. ELLA识别了具有明显亚细胞模式的基因,并将它们与mRNA特征联系起来.

    科学领域:

    • 分子生物学分子生物学
    • 计算生物学 计算生物学
    • 基因组学就是基因组学.

    背景情况:

    • 高分辨率的空间转录学使细胞下基因表达的测量成为可能.
    • 了解mRNA局部化对于细胞功能至关重要.

    研究的目的:

    • 引入亚细胞表达局部化分析 (ELLA) 用于建模亚细胞mRNA局部化.
    • 在高分辨率空间转录学中检测细胞内具有空间表达变异的基因.

    主要方法:

    • 开发了ELLA,一种使用统一蜂坐标系的计算方法.
    • 在空间计数数据建模中采用非均的Poisson过程.
    • 利用表达梯度函数来描述细胞下表达模式.

    主要成果:

    • 对于各种基因类型,ELLA确定了不同的亚细胞局部化模式.
    • 在核中丰富的基因通常是长的非编码RNA或蛋白质编码mRNA,基因长度较长.
    • 参与细胞质或膜活动的基因在这些细胞区中表现出丰富.
    • 揭示了动态的细胞循环依赖的亚细胞局部化模式.

    结论:

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  • 埃拉是一个强大的,坚固的,多功能工具,用于分析亚细胞空间表达的变化.
  • 该方法增强了对mRNA定位及其与基因特征的关系的理解.
  • 埃拉促进了对细胞周期期间动态基因表达的洞察.