丸特异性蛋白质WDR64通过与ODF1相互作用,参与手环和鞭毛的形成
Yunfei Zhang1,2, Xiaowei Xing2, Lihua Huang2
1Department of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.
Heliyon
|October 10, 2024
概括
这项研究确定了WDR64,一种对精子发育至关重要的新型蛋白质. WDR64与ODF1相互作用,在精子生成过程中在精子尾部组装中发挥关键作用.
科学领域:
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
背景情况:
- WD40重复 (WDR) 蛋白质对于蛋白质相互作用和细胞过程至关重要.
- 众所周知,WDR蛋白对于精子生成的各个阶段至关重要.
- 关于WDR64在男性生殖中的特定作用在很大程度上仍未被探索.
研究的目的:
- 为了识别和表征一种新的丸特异性WD40重复蛋白,WDR64.
- 调查精子生成过程中WDR64的表达模式和局部化.
- 阐明WDR64在精子发育中的分子功能和相互作用伙伴.
主要方法:
- 基因鉴定和序列分析.
- 逆转录聚合酶连锁反应 (RT-PCR) 和西方涂抹用于表达分析.
- 免疫光显微镜用于蛋白质定位.
- 免疫沉,然后进行质谱 (IP-MS) 来识别相互作用的蛋白质.
主要成果:
- 一个新的丸特异性基因,WDR64,编码WD40蛋白质,被确定并发现在哺乳动物中保存.
- WDR64在丸中表达高,在分娩后动态增加,在精子发育和成熟精子中表现出特定的局部化模式.
- 发现WDR64与ODF1相互作用,形成局部化到手环和精子尾部中间部的复合物,这表明它在手环和鞭子组装中的作用.
结论:
- WDR64是一种新型的丸特异性蛋白质,在精子生成中起着关键作用.
- 在精子形成过程中,WDR64/ODF1复合体参与了手环和鞭毛的结构组织.
- 这项研究提供了关于WDR64在男性性器官发育中的功能和机制的基础见解.
相关概念视频
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Mechanism of Lamellipodia Formation
2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Restarting Stalled Replication Forks
5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Flagella and Motility in Bacteria
3
Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
3
Fimbriae, Pili, and Axial Filaments
6
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
6


