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低频脉冲电磁场通过调节连xin 43 的表达来促进骨髓衍生中酶干细胞的骨质基因分化

Zhi-Jun Lu¹, Hou-Yun Gu¹, Zhi-Qiang Li¹

¹Department of Spine Surgery, Ganzhou People's Hospital (The Affiliated Ganzhou Hospital of Jiangxi Medical College of Nanchang University, Ganzhou Hospital-Nanfang Hospital of Southern Medical University), Ganzhou, Jiangxi 341000, P.R. China.

Experimental and therapeutic medicine

|October 10, 2024

概括

低频脉冲电磁场 (LPEMF) 增强骨髓介质干细胞 (BMSC) 的骨质分化. 这种效应是由连xin 43 (Cx43) 调节的介导,促进骨形成.

科学领域:

生物医学工程生物医学工程
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

介质细胞干细胞 (MSC) 对于骨再生至关重要.
连结素43 (Cx43) 在细胞沟通和分化中发挥作用.
低频脉冲电磁场 (LPEMF) 显示出刺激细胞过程的潜力.

研究的目的:

调查LPEMF对大鼠骨髓衍生中酶干细胞 (BMSCs) 的骨质分化的影响.
为了阐明连接素43 (Cx43) 在LPEMF诱导的骨质生成中的作用.

主要方法:

隔离和实验室培养大鼠BMSCs.
扩散的CCK-8测定,性酸酶 (ALP) 活性和骨质基因表达分析.
病毒载体介导的RNA干扰以抑制Cx43表达.
西部涂抹以确认Cx43表达水平.

主要成果:

80Hz的LPEMF在1小时内显著增强了BMSC增殖和骨质分化,由ALP活性,基因表达和阿里扎林红色S染色证实.
在LPEMF治疗期间,Cx43表达被上调.
抑制Cx43部分逆转了LPEMF诱导的骨质分化的增强.

结论:

80Hz的LPEMF在1小时内有效促进BMSCs的骨质性分化.
Cx43是LPEMF驱动的骨质生成过程中的关键媒介.
针对Cx43与LPEMF一起,可能为骨再生提供治疗策略.

关键词:

骨质损失导致骨质损失.骨髓的 stromal 细胞骨髓的 stromal 细胞连接器 43 连接器 43 低频中频脉冲脉冲低频中频脉冲骨质生成的差异化分化

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...