相关实验视频
Updated: Jun 11, 2025

11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
22.4K
低丸激素度提高了与塞尔托利细胞共同培养的山羊精子体干细胞的殖民化和活力
Hossein Salimi1, Peyman Rahimi Feyli1, Kheirollah Yari2
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Reproduction in domestic animals = Zuchthygiene
|October 10, 2024
概括
优化山羊精子干细胞 (SSC) 培养对于生殖技术至关重要. 60μg/mL的低丸激素度显著提高了与塞尔托利细胞共同培养中的SSC存活率和殖民地形成.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 动物科学动物科学
背景情况:
- 精子干细胞 (SSC) 对于通过自我更新和分化来维持精子生成至关重要.
- 羊SSC培养研究有限,阻碍了转基因动物生产等生殖技术的进步.
- 了解影响SSC在体外增殖的因素对于生殖细胞采购至关重要.
研究的目的:
- 为了研究不同度的丸激素对山羊SSC存活和殖民的影响,与塞尔托利细胞共同培养.
- 确定山羊SSC增殖的最佳条件,以在生殖生物技术中潜在应用.
- 评估丸激素在支持SSC维护和扩张中的作用.
主要方法:
- 在DMEM/F12介质中隔离并与Sertoli细胞共培养山羊SSC,并补充了生长因子.
- 培养物在10天内暴露在不同度的丸激素 (0,60,120,240μg/mL) 中.
- 通过免疫细胞化学和RT-PCR评估SSC存活率,殖民地数量和区域,以及标记物表达 (PGP9.5,ID-4,VASA,OCT-4).
主要成果:
- 对于山羊SSC殖民和生存能力的最佳丸激素度为60μg/mL.
- 与对照组相比,60μg/mL丸激素组的SSC殖民地数量显著增加 (p <0.05).
- 关键的生殖细胞标记物和性酸酶活性证实了SSC的存在和活力.
结论:
- 低丸激素度 (60微克/毫升) 是最佳的,可以增强山羊SSC殖民和共同培养系统中的生存.
- 这些发现有助于优化山羊SSC培养,以便在未来在辅助生殖和基因工程中的应用.
- 进一步的研究可以利用这些见解来改进山羊养殖和保护策略.
相关概念视频
Testosterone: Functions and Regulation
568
The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...
568
Spermatogenesis
102.2K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.2K
Infertility in Males
253
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
253
Testes: Histology
731
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
The spermatogenic cells, responsible for producing sperm, are...
731

