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克拉瓦塔3无敏受体激酶调节Arabidopsis中的侧向根启动和间隔

Xianghu Meng¹, Rui Ye¹, Jing Cao¹

¹Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.

Plant physiology

|October 10, 2024

概括

CLAVATA3无敏受体关节 (CIKs) 和类似受体关节7 (RLK7) 感知到LBD SIXTEEN 2 (TOLS2) 信号的目标. 这种相互作用通过MKK4/5-MPK3/6-PUCHI通路调节横向根启动和间距.

科学领域:

植物生物学植物生物学
分子植物科学分子植物科学
发育生物学发展生物学

背景情况:

侧根 (LR) 发育对于植物适应环境变化至关重要.
类似受体的KINASE 7 (RLK7) 和它的连接体TARGET OF LBD SIXTEEN 2 (TOLS2) 是已知的LR启动和间隔的调节者.
对于RLK7对TOLS2信号感知和转导的精确分子机制尚不完全了解.

研究的目的:

调查CLAVATA3无敏受体激酶 (CIKs) 在Arabidopsis横向根部发育中的作用.
阐明CIK与RLK7介导的信号通路在调节LR启动和间隔方面的相互作用.

主要方法:

对Arabidopsis thaliana中的各种cik突变体的表型分析.
生物化学测试检查CIK,RLK7和其他信号组件之间的相互作用.
在CIC突变体中分析TOLS2的敏感性.
研究奥素,PUCHI,MKK4和MKK5在LR发育中的作用.

主要成果:

高阶cik突变体表现出增加的LR启动和缺陷的LR间距,对TOLS2.2的敏感性降低.
托尔斯2应用增强了CIK-RLK7相互作用和RLK7依赖的CIK酸化.
过度表达PUCHI和激活MKK4/MKK5部分挽救了cik和rlk7-3突变体中的LR间距缺陷.
素最大影响了CIK亚细胞局部化,确定了横向根创始细胞.

结论:

CIK和RLK7协同感知TOLS2信号,调节LR启动和间隔.
该信号通路涉及MK4/5,MPK3/6和转录因子PUCHI.
由auxin调节的CIK亚细胞局部化,在指定横向根创始细胞方面发挥着作用.

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