假定的4型分泌系统效应器BSPD参与维持病原体布鲁塞拉病原体的包裹完整性
Maren Ketterer1, Petra Chiquet1, Mara Esposito1
1Biozentrum, University of Basel, Basel, Switzerland.
mSphere
|October 10, 2024
概括
布鲁塞拉堕胎的外膜完整性是由BSPD蛋白维持的,这对于在压力条件下和宿主细胞内的生存至关重要. 这一发现增强了对布鲁塞洛斯病原体的理解.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 血病是一种重要的动物传染病,由细胞内病原体*Brucella*引起.
- 了解细菌适应宿主环境是对抗感染的关键.
- 细菌外对于病原体的生存和毒性至关重要.
研究的目的:
- 为了研究保存蛋白BspD在*Brucella abortus*包裹完整性中的作用.
- 为了确定BSPD的存在是否与Rhizobiales.com内的特定生活方式或分泌系统有关.
- 评估BSPD对*Brucella*在压力下和宿主细胞内的生存的影响.
主要方法:
- 在Rhizobiales中对BSPD保护的生物信息分析.
- 在布鲁塞拉流产*中*bspD*基因的遗传删除.
- 在静止阶段和EDTA暴露下对*bspD*突变体的表型分析.
- 巨细胞感染模型,以评估细胞内生存和微殖民地形成.
主要成果:
- BspD是Rhizobiales中保存的蛋白质,不会与T4SS或特定的生活方式共同演变.
- 删除*bspD*导致信封完整性受到损害和细菌形态异常.
- *bspD*突变体在巨细胞中表现出减少的细胞内微殖民地形成.
- BspD对于*B. abortus*包的完整性至关重要,尤其是在压力下.
结论:
- 在保持布鲁塞拉信封的完整性方面,BSPD起着至关重要的作用,特别是在压力下.
- 这一功能对于有效的细胞内生存和宿主细胞内的殖民是必不可少的.
- 这些发现有助于了解布鲁塞拉病原体,并可能指导未来的治疗干预措施.
相关概念视频
Gram-negative Bacterial Protein Secretion Systems
1
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1
Outer Layers of the Cell Envelope
3
The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers of polysaccharides that firmly attach to the bacterial cell wall. These structures serve as formidable protective barriers, preventing...
3
Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Formation of Lipopolysaccharides
1
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
1
Bacterial Signaling
31.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
31.6K
Fimbriae, Pili, and Axial Filaments
6
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
6


