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与两个主要的格拉姆阳性病原体毒性调节蛋白中的碳催化剂抑制相关的新型动机

Jerry K K Woo¹, Adriana M Zimnicka¹, Michael J Federle¹

¹Department of Biopharmaceutical Sciences, Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA.

Microbiology spectrum

|October 10, 2024

概括

这项研究揭示了一种新的机制,它将细菌的碳代谢与毒性联系起来. 在MGA和ProfA调节器中酸化特定的氨酸控制了像Streptococcus pyogenes这样的病原体中的毒性基因表达.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌病原体的产生

背景情况:

碳催化剂抑制 (CCR) 是一种调节碳水化合物利用的保存过程.
CCR可以是CcpA依赖的或CcpA独立的,通常涉及具有光转移酶调节域 (PRD) 的转录调节器.
含有PRD的调节剂与转移酶系统 (PTS) 组件相互作用,其中保留的histidine和cysteine残留物可能参与酸化.

研究的目的:

为了研究一个新型的囊含有基因在MGAGA的作用,一个毒性调节器的Streptococcus pyogenes,在碳催化剂抑制 (CCR).
探索细胞代谢和病毒性基因表达之间的潜在联系,通过在MGA和PrfA中对氨酸残留物进行翻译后修饰.
为了确定Magas的氨酸残留的素捐赠者.

主要方法:

局部导向的突变发生,以在MGA中产生缩性和模仿性氨酸替代物.
评估Rgg2/3定数感应系统中CCR修改的情况.
基因分析以确定素捐赠者,可能是ManL (曼诺斯PTS系统EIIA/B子单元).
在Listeria monocytogenes的PrfA上进行了类似的突变发生和分析.

主要成果:

在Maga的缩和模仿氨酸替代物显著改变了Rgg2/3系统的CCR.
遗传证据表明,ManL是Magas中的氨酸残留物的素捐赠者.
在PrfA中类似的囊替代也改变了PrfA依赖的毒性基因表达的CCR.
在 Mga 和 PrfA 中,一种新的,共享的含氨酸的动机将碳代谢与毒性联系起来.

结论:

在Maga和PrfA中保存的氨酸残留物的酸化代表了连接细菌代谢和毒性的一种新机制.
这种在细菌转录调节器中对氨酸的翻译后修饰是一种以前研究不足的现象.
这些发现表明,一种新的监管层可以控制阳性病原体的毒性.

关键词:

在CCR的基础上,李斯特里亚单细胞原生菌 (Listeria monocytogenes) 是一种马格拉马格拉是什么意思这就是为什么PrfAA.链球菌是一种链球菌.毒性毒性是一种毒性.

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