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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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条形码 100K 标本:在单个纳米孔运行中运行

Paul D N Hebert1, Robin Floyd1, Saeideh Jafarpour1

  • 1Centre for Biodiversity Genomics, University of Guelph, Guelph, Ontario, Canada.

Molecular ecology resources
|October 10, 2024
PubMed
概括

DNA 条形码推进了生物圈管理的物种识别. 新的纳米孔测序技术大大降低了成本,使得大规模的动物物种普查可行,每标本0.01美元.

科学领域:

  • 生物多样性研究的研究.
  • 基因组技术 基因组技术
  • 保护科学 保护科学

背景情况:

  • 全球生物圈管理需要全面的物种数据,但高成本限制了数据采集.
  • DNA条形码通过BIN系统自动识别动物物种,但需要负担得起的测序.
  • 之前的高通量测序平台很昂贵,限制了可访问性.

研究的目的:

  • 评估使用牛津纳米孔技术 (ONT) 测序器用于大规模DNA条形码的成本效益和可行性.
  • 为了证明最近在ONT流细胞技术的进步使生物多样性研究能够实现高通量,低成本的DNA测序.

主要方法:

  • 利用ONT MiniION和Flongle流细胞进行聚合的安普利康的DNA测序.
  • 具有特征的安普利康池代表每个MinION流量细胞多达10万个标本,每个Flongle流量细胞多达数千个标本.
  • 计算了DNA测序步骤的每个样本的成本.

主要成果:

  • ONT最新的R10.4.1流量电池提供了更好的保真度,克服了以前的采用障碍.
  • 一个单一的MinION流细胞成功地描述了来自10万个标本的集合样本.
  • 一个Flongle流细胞处理了几千个标本的聚合样本.
关键词:
生物信息学/生物信息学/物理信息学DNA 条形码编码安普利康序列的测序方法昆虫 昆虫 是一种昆虫.分子进化分子演变.

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  • 每个样本的DNA测序成本降至0.01美元,成为工作流程中最便宜的步骤.

    • 结论:

    • 牛津纳米孔测序技术,特别是R10.4.1流细胞,为大规模DNA条形码提供了经济有效的解决方案.
    • 这种技术进步显著降低了对进行全面物种普查的障碍,有助于生物圈管理.
    • 现在,DNA测序在经济上可行,用于分析完成动物物种清单所需的数十亿个标本.