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活性化物和表面活性剂的协同作用,以加强对微型污染的控制
Sudhir K Shukla1,2, T Subba Rao3, Malathy N4
1Biofouling and Biofilm Processes Section, Water & Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam, 603102, India. skshukla@igcar.gov.in.
Archives of microbiology
|October 10, 2024
概括
仅活性化物对冷却系统中的细菌和生物膜的有效性有限. 将其与Triton-X 100等表面活性剂相结合,可以显著增强生物膜分散和杀死细菌.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 工业生物技术 工业生物技术
背景情况:
- 生物膜,即细胞外聚合物物质 (EPS) 基质中的微生物群落,在工业冷却系统中存在问题.
- 核电厂使用活性生物制剂来控制冷凝器冷却系统中的生物生长.
- 海水冷却系统面临着浮游生物和生物膜形成细菌的挑战.
研究的目的:
- 评估活性胺对常见海水细菌的抗菌和抗生物膜疗效.
- 评估表面活性剂Triton-X 100对活性胺的疗效的影响.
- 确定在发电厂冷却系统中控制微污染的最佳策略.
主要方法:
- 在1ppm时测试了活性胺对浮游生物Pseudomonas aeruginosa的疗效.
- 用Triton-X 100评估细菌细胞表面的疏水性变化.
- 进行了生物膜抑制试验和光显微镜,使用活/死染色.
主要成果:
- 活性化物在1ppm显示最小的杀死浮游生物P. aeruginosa.
- 特里顿-X 100降低了细胞的疏水性,增加了对活性胺的敏感性.
- 1 ppm活性胺具有有限的生物膜抑制,但5-10 ppm显示出显著的抑制.
- 1 ppm活性化物和Triton-X 100协同分散的预制生物膜的组合.
- 显微镜检查证实,在用这种组合治疗的生物膜中,细胞死亡量很大.
结论:
- 仅仅活性化物可能不足以有效控制海水冷却系统中的微污染.
- 生物相容的表面活性剂可以通过破坏生物膜来显著提高活性化物治疗的疗效.
- 组合疗法为改善工业环境中的生物膜管理提供了一个有希望的策略.
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