从早期胚胎和子宫内膜之间的交叉来源的小细胞外囊泡可能会介导白色体功能†
Alessandra Bridi1, Juliano Rodrigues Sangalli1, Ricardo Perecin Nociti1
1Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, 13635-900, Pirassununga, Brazil.
Biology of reproduction
|October 10, 2024
概括
来自牛胚胎的小细胞外囊 (sEV) 重新编程子宫内膜,改变基因表达. 这些携带不同的miRNA载荷的sEVs影响黄体的功能,影响怀孕的成功.
科学领域:
- 生殖生物学 生殖生物学
- 细胞外囊泡 细胞外囊泡
- 胚胎与母亲的相互作用
背景情况:
- 早期的胚胎与母亲的沟通对于成功怀孕至关重要.
- 微小的细胞外囊泡 (sEVs) 调解这些相互作用.
- 胚胎起源 (体内或体外) 影响sEV货物.
研究的目的:
- 研究在体内和体外生产的牛胚胎如何影响子宫内膜基因表达.
- 分析由不同胚胎培养的子宫内膜扩展体释放的sEVs的miRNA含量.
- 为了确定这些胚胎衍生的sEVs对红体功能的影响.
主要方法:
- 子宫内膜扩展物的共同培养与体内 (AI) 或体外 (IVF) 牛胚胎.
- 从条件介质中回收和描述SEV.
- 对子宫内膜和子宫外膜扩展物的转录和miRNA分析.
- 不同基因和miRNA表达分析.
主要成果:
- 胚胎的存在和起源显著改变了子宫内膜基因表达 (分别是45和211个基因).
- 在体内与体外胚胎共同培养的子宫内膜扩展体的sEV显示出不同的miRNA配置文件 (4miRNAs).
- 暴露于这些sEV诱导了体扩增基因和miRNA表达 (1360个转录,15个miRNA) 的显著变化.
结论:
- 牛胚胎起源 (体内/体外) 诱导子宫内膜中明显的分子变化.
- 胚胎和子宫内膜衍生的SEV携带特定的miRNA载荷,调节红色体的功能.
- 通过sEV介导的沟通是调节红色体生存能力的关键机制,用于成功怀孕.
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