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光子微生物阵列数字时间分辨率光超敏感平台,用于同时检测多种真菌毒素

Yuting Zhu¹, Wei Li², Jing Yang¹

¹School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.

Analytical chemistry

|October 10, 2024

概括

一个新的时间解析光免疫测试 (TRFIA) 平台,在光子微珠阵列上使用欧欧纳米颗粒,提供高度敏感的多目标真菌毒素检测. 这一进步显著改善了快速食品安全查的传统方法.

科学领域:

分析化学分析化学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

侧流免疫检测在多目标查中面临敏感度,线性范围和交叉反应性的限制.
食品中毒素污染对健康构成重大风险,需要敏感和快速检测方法.

研究的目的:

开发一种新的时间解析光免疫测试 (TRFIA) 平台,用于敏感,多重检测真菌毒素.
克服现有的免疫测试技术的局限性,以快速检查食品安全.

主要方法:

结合欧酸聚乙烯 (PS@Eu) 纳米颗粒与用于真菌毒素检测的单克隆抗体.
在光子微珠阵列 (PMA) 上使用具有竞争力的TRFIA协议,使用数字时间分辨率光读取器.
评估性能指标,包括线性检测范围,检测极限 (LOD),粒样中的特异性,回收和可重复性.

主要成果:

开发的TRFIA显示了广泛的线性检测范围 (DON的0.01-1000 ng/mL,OTA的0.1-100 ng/mL,AFB1的0.01-100 ng/mL) 和低的LOD (DON的7.9 pg/mL,OTA的18 pg/mL,AFB1的7.7 pg/mL).
光子微珠阵列 (PMA) 由于增加了电场分布,使光信号大约增强了两倍.
新的TRFIA比传统的TRFIA敏感1000倍,在谷物样本中表现出良好的特异性,回收率 (76.68-117.26%) 和可重复性.

结论:

新的TRFIA平台有效地克服了传统免疫试验在多菌毒素检测方面的局限性.
该平台具有高灵敏度,广泛的线性范围,在真实样本中表现出色,这表明快速食品安全查的巨大潜力.
来自PMA的增强光有助于这种先进的免疫测试系统的优越灵敏度.

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