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基于四面体DNA框架的酶循环激活生物传感器,用于精确的瘤in situ分子成像

Muchun Yu¹, Yingyu Zhang², Mengxin Zhang¹

¹Health Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, China.

|October 10, 2024

概括

这项研究引入了一种新的DNA生物传感器,用于精确的瘤成像. 激活apurinic/apyrimidinic内核酶1 (APE1) 的传感器具有高灵敏度和特异性,使瘤细胞的分化和风险分类成为可能.

科学领域:

生物医学工程生物医学工程
分子成像学分子成像学
核酸纳米技术核酸纳米技术

背景情况:

瘤分子成像需要具有高空间特异性和灵敏度的策略,以克服诸如瘤外信号泄漏和低生物标志物丰度等挑战.
酶apurinic/apyrimidinic endonuclease 1 (APE1) 是一个有前途的目标,因为它在癌细胞 (细胞质和核) 中与正常细胞 (主要是核) 相比表达的差异很大.

研究的目的:

开发一种循环激活的酶生物传感器,用于瘤细胞特异性分子成像.
利用apurinic/apyrimidinic内核酶1 (APE1) 酶的活性,提高检测瘤细胞的灵敏度和空间特异性.

主要方法:

一个四面体框架核酸 (tFNA) 经过基底 (AP) 位点 (AP-tFNA) 修改,被设计为APE1.1的基底.
生物传感器依赖于APE1诱导的AP点的裂变,将光体和火器分开以产生可检测的光信号.
循环激活机制允许在重复APE1识别和裂变时进行信号放大.

主要成果:

AP-tFNA生物传感器在瘤细胞与正常细胞的分化方面表现出高的特异性,无论是体外还是体内.
该系统实现了对瘤细胞的敏感检测,与APE1酶活性相关.
生物传感器成功监测了神经母细胞细胞中的耐药性,并使用血样本对患者的风险进行了分类.

结论:

开发的AP-tFNA生物传感器为瘤分子成像提供了一个敏感和空间特定的平台.
这种方法有可能实时监测癌症患者的治疗反应和临床风险分层.
循环激活酶策略为改善瘤学诊断能力提供了一个有希望的途径.

关键词:

在APE1中,APE1是APE1.生物传感器生物传感器临床样本的临床样本.影像成像技术影像成像技术神经母细胞瘤的神经母细胞瘤

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