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使用PT-SPE和HPLC-MS/MS的Cis-diol-microporous有机网络在人类尿液中增强检测甲基胺

Rong Chen¹, Yehong Han², Ligai Bai²

¹Hebei Key Laboratory of Public Health Safety, College of Public Health, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, China.

Journal of chromatography. A

|October 10, 2024

概括

一种新的微孔有机网 (MONs-2OH) 材料有效吸附甲基醇胺 (CA). 这使得一种敏感的方法可以在尿液中检测CA,帮助诊断抑郁症.

科学领域:

材料科学材料科学材料科学
分析化学分析化学
生物化学生物化学

背景情况:

甲基醇胺 (CA) 是神经疾病的关键生物标志物.
对于辅助诊断,需要敏感和高效的CA检测方法.
目前的方法在灵敏度,选择性和样本准备方面面临挑战.

研究的目的:

为了合成和表征一种新的微孔有机网络 (MONs-2OH) 材料.
开发一种灵敏,高效的方法来检测人类尿液中的甲醇胺.
为了评估MONs-2OH在管管尖固体相提取 (PT-SPE) 中的性能,用于CA分析.

主要方法:

通过室温和Sonogashira合反应合成MONs-2OH.
将MONs-2OH纳入管管尖固体相提取 (PT-SPE) 中.
使用高性能液体染色学-质谱学/质谱学 (HPLC-MS/MS) 检测人类尿液中的CA.

主要成果:

由于结和π-π堆叠,MONs-2OH对CA具有出色的吸附特性.
开发的PT-SPE-HPLC-MS/MS方法显示了广泛的线性范围 (0.5-500 ng/mL) 和低的检测极限 (0.06-0.26 ng/mL).
与商业吸附剂相比,MONs-2OH显示出更高的吸附能力和回收能力.

结论:

MONs-2OH 是一种高度稳定和高效的CAS吸收剂.
新的PT-SPE方法提供了快速,灵敏和准确的CA确定.
这种方法对抑郁症的辅助诊断和先进的样本预处理技术具有重大潜力.

关键词:

吸附机制的吸附机制是什么卡特霍胺的检测方法在HPLC-MS/MS分析中,微孔的有机网络是微孔的有机网络.管管尖的固体相提取方法

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