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扩散型大B细胞淋巴瘤中单细胞测序:C1qC是一种潜在的瘤促进因子

Guangcan Gao¹, Naitong Sun², Yaping Zhang³

¹Department of Oncology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China; Nantong University Medical School, 19 Qixiu Road, Nantong 226001, Jiangsu, China; Department of Clinical Biobank & Institute of Oncology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.

International immunopharmacology

|October 10, 2024

概括

补充成分1q (C1q) 通过增强M2巨细胞活动,促进扩散性大B细胞淋巴瘤 (DLBCL). 准C1qC为DLBCL患者提供了潜在的治疗策略.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
分子生物学分子生物学

背景情况:

补充元件1q (C1q) 是经典补充路径的组成部分.
较高的C1q表达与各种癌症的预后不佳相关.
C1q在扩散性大B细胞淋巴瘤 (DLBCL) 病原发生中的特定作用尚不清楚.

研究的目的:

研究C1q促进DLBCL的机制.
为了确定与DLBCL预后相关的免疫细胞子组.
探索C1qC作为DLBCL的潜在治疗点.

主要方法:

多重复合免疫组织化学 (mIHC) 和单细胞测序用于分析DLBCL组织中的免疫细胞群和C1qC表达.
通过Lasso回归来构建基于免疫细胞的风险预测模型.
在C1qC敲击后进行体外共同培养实验和分子测定 (西方斑点,qPCR) 来评估M2巨细胞的功能和与淋巴瘤细胞的相互作用.
对DLBCL患者的临床数据进行分析,以评估C1qC+ M2巨的预后意义.

主要成果:

在DLBCL中,T细胞亚型,中性粒细胞和M2巨细胞被确定为预后因素.
C1qC被确定为与DLBCL预后相关的关键基因,在M2巨细胞中观察到高表达.
抑制C1qC降低了M2巨细胞标记物 (例如CD163),并降低了它们促进淋巴瘤细胞增殖和降低药物敏感性的能力.
高表达C1qC+M2巨细胞独立预测DLBCL患者的预后不佳.
增加的C1qC表达与免疫检查点激活,调节性T细胞 (Treg) 透和M2巨丰富相关.

结论:

C1qC在DLBCL病变发生过程中起着重要作用,主要是通过其对M2巨细胞的影响.
C1qC+M2巨细胞作为DLBCL的潜在预后生物标志物.
准C1qC为DLBCL治疗提供了一个有希望的治疗途径.

关键词:

C1qCC 的意思是 DLBCL DLBCL 是一个字母.在M2巨细胞中.在此之前,TME的 mIHC 的使用情况.

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