在Prototheria中的eicosanoid生物合成酶.
Kumar R Kakularam1, Eda Gündem1, Sabine Stehling1
1Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt Universität zu Berlin, Department of Biochemistry, Charitéplatz 1, D-10117 Berlin, Germany.
Biochimica et biophysica acta. Molecular and cell biology of lipids
|October 10, 2024
概括
古代哺乳动物具有活跃的eicosanoid代谢,表达功能ALOX15B,但缺乏ALOX15基因. 这表明ALOX15是在Prototheria-Metatheria分裂后从ALOX15B进化出来的.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 伊可索诺伊德是细胞功能和疾病发病过程中至关重要的脂质媒介.
- 哺乳动物的eicosanoid生物合成在先进物种中得到了很好的研究,但在古代哺乳动物 (Prototheria) 中了解得很少.
研究的目的:
- 在Prototheria中调查脂氧酶 (ALOX) 和前列腺素合成酶 (PTGS) 基因的存在和功能.
- 在哺乳动物中描述ALOX15和ALOX15B基因的进化起源.
主要方法:
- 对于ALOX和PTGS基因,鸟和鱼的基因组分析.
- 原始体ALOX15B异构体的表达和功能特征.
- 酶活性与使用突变发生的人类和小鼠正义学者的比较.
主要成果:
- 原生动物的基因组包含完整的ALOX5,ALOX12,ALOX12B,ALOXE3,PTGS1和PTGS2基因.
- 确定了两个ALOX15B基因,其中一个在鱼中受损;ALOX15基因缺席.
- 原始体ALOX15B异型表现出保留的反应特异性,而Jisaka决定性修饰改变了催化特性.
结论:
- 原生菌表现出具有功能ALOX15B正义基因的活性eicosanoid代谢,但缺乏ALOX15基因.
- ALOX15很可能源于在Prototheria-Metatheria过渡期间的ALOX15B基因复制.
- 这项研究揭示了早期哺乳动物脂质媒介的演变.
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