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Updated: Jun 10, 2025

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
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PAR-dCLIP:通过结合分离步骤,可以检测与5'末端结合的RNA结合蛋白标转录

Samantha Lisy1, Katherine Rothamel2, Yelena Perevalova-Pinzul1

  • 1Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, United States.

Methods in enzymology
|October 10, 2024
PubMed
概括

我们介绍了可光激活的核糖核酸增强交叉链接和免疫沉降与切断 (PAR-dCLIP),一种研究与5'封闭RNA转录相互作用的RNA结合蛋白 (RBPs) 的方法. 这种技术通过包括分离步骤来增强RNA测序和RBP分析.

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