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初级内牙感染中的微生物多样性:人口统计和放射特征
David Schuweiler1, Ronald Ordinola-Zapata2, Matthew Dietz3
1Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, 55455, USA.
Clinical oral investigations
|October 10, 2024
概括
根管微生物组在内牙感染中没有受到常见的临床因素的显著改变. 然而,较大的周周病变与不同的微生物群体组成有关,这表明病变大小和口腔细菌之间存在联系.
科学领域:
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
- 口腔健康 口腔健康
背景情况:
- 根管微生物组在内牙感染的发展和持续中起着至关重要的作用.
- 了解影响这种微生物群的因素对于有效的治疗策略至关重要.
研究的目的:
- 调查微生物群体组成在初级内牙感染和临床或放射性因素之间的关联.
- 为了确定临床表现或病变大小是否会影响受感染根管内的微生物概况.
主要方法:
- 16S rRNA amplicon测序被用来描述71名患有初级内牙感染的患者的微生物组.
- 收集了临床数据 (疼痛,鼻道,,探测深度,年龄,性别) 和放射数据 (周围指数 - PAI).
- 使用Shannon和Chao1指数评估阿尔法多样性;使用ANOSIM分析社区组成差异.
主要成果:
- 没有发现微生物组合的显著差异,这些差异涉及到临床因素,如打击痛或鼻道存在.
- 微生物组多样性 (α多样性) 在低 (PAI ≤2) 和高 (PAI =5) 放射性损伤类别之间没有显著差异.
- 社区的组成受到周周指数 (PAI) 的显著影响,较大的病变显示了Prevotellaceae,Olsenella,Oribacterium,Selenomonadaceae spp.和Treponema的丰度增加.
结论:
- 通常在顶端牙周炎中评估的临床因素对根运河微生物群的影响有限.
- 根管微生物组的组成受到角放射性病变的存在和大小的显著影响.
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