菌-真菌共同培养:哪个合作伙伴有助于抗真菌活性?
Wendy A Stirk1, Bernadett Pap2, Gergely Maróti2,3
1Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg Campus, P/Bag X01, Scottsville, Pietermaritzburg, 3209, South Africa. stirk@ukzn.ac.za.
Current microbiology
|October 10, 2024
概括
菌显示出生物杀虫剂的抗真菌潜力. 与真菌共同培养Nostoc菌株揭示了特定的抗真菌活性,突出了确定准确生物活性评估的必要性.
科学领域:
- 微生物学 微生物学
- 农业科学 农业科学
- 生物技术是生物技术.
背景情况:
- 菌产生具有抗真菌性能的二次代谢物,为农业中的环保生物杀虫剂提供了潜力.
- 选蓝菌菌株的生物活性往往使用非菌培养,这可能会影响观察到的结果.
- 了解在蓝藻菌种中真菌同隔离物的影响对于准确的生物活性评估至关重要.
研究的目的:
- 为了研究两种蓝菌菌株 (Nostoc muscorum MACC-189和N. linckia MACC-612) 与特定的真菌伙伴共同隔离的体外抗真菌活性.
- 为了确定观察到的抗真菌活性是源于蓝藻细菌还是其真菌同隔离物.
- 评估真菌同居者对蓝菌种植物的生物活性的影响.
主要方法:
- 分离和鉴定真菌的共同伙伴 (Purpureocillium lilacinum和Sarocladium sp.) 的情况. 来自蓝藻细菌菌株培养物.
- 在实验室测试蓝藻菌-真菌共同培养和隔离的真菌合作伙伴,对七种植物病原体进行测试.
- 在化10天后对抗真菌作用的半定量评估.
主要成果:
- 菌-真菌共同培养对Monilinia fructigena和Aspergillus sp.表现出抗真菌活性.
- 在Nostoc muscorum/Purpureocillium lilacinum共同培养中,表现出最显著的抗真菌作用.
- 紫罗兰 (Purpureocillium lilacinum) 以剂量依赖的方式抑制了Monilinia fructigena,而没有一种真菌分离物抑制了Aspergillus sp.
- 结果表明,真菌伴侣对M. fructigena进行抗真菌活性,而蓝菌伴侣对Aspergillus sp.进行抗真菌活性.
结论:
- 存在的真菌同隔离物显著影响了观测到的菌培养的抗真菌活性.
- 准确评估蓝藻细菌的生物活性需要使用菌培养物或彻底识别所有共存的生物体.
- 这项研究强调了在生物勘探中考虑微生物社区组成对于新型生物杀虫剂从蓝藻细菌的重要性.
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