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Delaney K Sullivan1,2, Kyung Hoi Joseph Min3, Kristján Eldjárn Hjörleifsson4

  • 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Nature protocols
|October 10, 2024
PubMed
概括

本研究介绍了 kallisto,bustools 和 kb-python,这是用于RNA测序 (RNA-seq) 分析的免费软件. 这些工具有效地从单个细胞或组织的原始测序数据中量化基因表达.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...