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GhMAC3e参与植物生长和对Verticillium dahliae的防御反应

Zhenghong Han¹, Yuanyuan Qiu¹, Ting Pan¹

¹State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Collaborative Innovation Center for Modern Crop Production Cosponsored by Jiangsu Province and Ministry of Education, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.

Plant cell reports

|October 10, 2024

概括

棉花基因GhMAC3e通过影响辅酶分布来影响植物生长,并通过调解酸信号来增强对Verticillium枯的抵抗力.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
植物生理学植物生理学

背景情况:

MOS4关联复合体 (MAC) 对于mRNA前拼接,拼接酶组合和植物免疫是至关重要的.
在MAC中,MAC3A/B,U-box E3泛素酶对植物发育,应激反应和抗病能力至关重要.
棉花中MAC3基因的特定作用,特别是GhMAC3e,在很大程度上是未被探索的.

研究的目的:

为了从棉花中克隆 GhMAC3e 基因.
调查棉花和Arabidopsis中的GhMAC3e的生物功能.
为了阐明GhMAC3e在植物生长,应激反应和垂直的衰退抵抗中的作用.

主要方法:

病毒诱导的基因沉默 (VIGS) 在棉花中抑制GhMAC3e表达.
在Arabidopsis.中转基因过度表达GhMAC3e.
对基因表达,激素含量和植物生长参数的分析.
在转基因阿拉比多普西斯中评估Verticillium枯耐药性.

主要成果:

GhMAC3e的表达是由各种压力 (盐,Verticillium dahliae,PEG) 和激素 (ABA,ETH,GA3,MeJA,SA) 诱导的.
制棉花中的GhMAC3e减少了生长,生物质和辅酶含量,改变了与生长相关的基因的表达.
在Arabidopsis中过度表达GhMAC3e增强了对Verticillium枯的抵抗力,显著上调PR1表达.

结论:

GhMAC3e通过影响辅酶分布和与生长相关基因的表达来调节棉花的生长.
GhMAC3e通过调解酸信号传递,独立于NPR1转录水平,在Verticillium枯耐药性中发挥着重要作用.

关键词:

这种植物名为Verticillium dahliae.一个辅助的Auxin.棉花棉花棉花棉花棉花在GhMAC3e中使用.这是MOS4相关复合体.

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