在Schizochytrium sp.中增强脂肪酸和omega-3的产生. 使用开发的安全港口表达系统
Ae Jin Ryu1, Won-Sub Shin1, Sunghoon Jang1
1CJ BIO Research Institute , CJ CheilJedang, Suwon-si, Gyeonggi- do, 16495, Republic of Korea.
Journal of biological engineering
|October 10, 2024
概括
我们在精神分裂症中开发了一种安全港的基因表达系统,通过向基因整合,提高了25%的多可萨赫酸 (DHA) 产量. 这种方法可以改善脂质的产生,并维持细胞的生长,用于工业应用.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 一种油性海洋微生物Schizochytrium具有生产生物燃料和omega-3脂肪酸的潜力.
- 有限的基因工程工具阻碍了Schizochytrium的工业脂质生产能力.
研究的目的:
- 开发一种稳定高效的基因工程系统,用于治疗精神分裂症.
- 为了增强脂质和多可沙赫酸 (DHA) 产量在精神分裂症.
主要方法:
- 开发了一个安全港的转基因表达系统,以克服位置效应和不稳定性.
- 鉴定了HRsite 2,在细胞染色体c的上游,作为同源重组的高效地点.
- 集成了HRsite 2的3-ketoacyl-ACP减少酶 (KR) 基因,用于向基因表达.
主要成果:
- HRsite 2显示了增强的sfGFP表达和同源重组效率.
- 在HRsite 2的KR基因表达显著增加了脂质和DHA的产生.
- 与野生类型的精神分裂症相比,转化剂的DHA含量增加了25%,增长稳定,葡萄糖利用.
结论:
- 通过使用安全港口网站在Schizochytrium建立了一个强大的同源重组系统.
- 在安全港区的目标KR基因表达增强了DHA的产生,而不会影响生长.
- 这一进步使得脂质衍生产品在思索中能够得到更有效的生物技术应用.
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