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细胞类型的特征和下器官的多样性

Narciso Pavon^1,2, Yubing Sun³, ChangHui Pak¹

¹Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, United States.

Frontiers in genetics

|October 11, 2024

概括

本综述比较了生成下器官的协议,重点是中等脊状神经元 (MSN) 和内神经元 (IN). 了解这些GABAergic神经元有助于神经疾病研究和临床转化.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
干细胞生物学干细胞生物学

背景情况:

神经器官对研究大脑发育和神经疾病至关重要.
为了产生特定区域的神经器官,存在不同的协议,从而产生不同的表型.
从修改的协议中验证细胞类型对研究人员来说是一个重大挑战.

研究的目的:

系统地比较现有的生成下特定器官的协议.
评估GABAergic神经元亚型的多样性,特别是通过这些协议产生的中等脊状神经元 (MSN) 和内神经元 (IN).
评估模式因素的体外适用性,并讨论特定区域神经器官发育的挑战.

主要方法:

系统的文献审查已发表的下器官协议.
对专注于GABAergic神经元生成 (MSNs和INs) 的方法的比较分析.
评估细胞类型多样性和用于有机体生成的分子途径.

主要成果:

通过不同的下有机体协议识别了形态学,基因表达和细胞群的变异.
详细比较生成特定GABAergic神经元亚型 (MSNs和INs) 的协议.
评估各种模式因素在指导神经器官发展方面的有效性.

结论:

为可靠的研究和疾病建模,对下器官进行标准化协议至关重要.
了解体外MSNs和INs的生成是推动神经系统疾病研究的关键.
改善特定区域神经器官的模式,有望为未来的临床转化提供希望.

关键词:

这些是MSN的MSN.大脑区域特定的有机体在FGF的基础上.这些是iPSCs.神经器官有机物神经器官有机物图案设计图案设计沙沙沙沙沙沙沙沙沙沙沙沙沙沙沙沙沙沙这里是subpallium的底层.

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