相关实验视频

Updated: Jun 10, 2025

Isolation, Transfection, and Culture of Primary Human Monocytes

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

解读内皮粒细胞巨-CSF在与艾滋病毒相关的慢性炎症中的作用

Soumya Panigrahi¹, Elizabeth Mayne², Susan Louw³

¹Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

|October 11, 2024

概括

在HIV患者中,微生物产品增加葡萄糖吸收和粒细胞巨细胞殖民地刺激因子 (GM-CSF),导致内皮功能障碍. -葡萄糖共传输体-2 (SGLT2) 抑制剂可以通过减少这种炎症来提供治疗效益.

科学领域:

心血管生物学心血管生物学
传染病免疫学传染病免疫学
内皮细胞生物学内皮细胞生物学

背景情况:

艾滋病毒感染者 (PWH) 往往由于慢性炎症和肠道屏障损伤导致内皮功能障碍 (ED).
在PWH中将微生物转移与ED联系起来的特定分子途径尚未完全理解.

研究的目的:

研究葡萄糖吸收和粒细胞巨细胞殖民地刺激因子 (GM-CSF) 在与艾滋病毒相关的内皮功能障碍中的作用.
探索-葡萄糖共运输体-2 (SGLT2) 抑制剂在缓解这些途径方面的治疗潜力.

主要方法:

在PWH的大动脉内皮质中评估了GM-CSF,托尔类受体-4 (TLR4) 和髓氧化酶的表达.
暴露初级人体内皮细胞的脂聚糖 (LPS) 来模仿细菌转位效应.
评估了SGLT2抑制剂对LPS激活细胞中的葡萄糖吸收,GM-CSF释放和ED的影响.
在SGLT2抑制剂的PWH中比较血GM-CSF度,而不是在药物上.

主要成果:

PWH显示GM-CSF,TLR4和髓氧化酶的大动脉内皮表达增加.
低血压刺激增加了内皮细胞中的葡萄糖吸收和GM-CSF表达.
在LPS激活细胞中,SGLT2抑制剂降低了葡萄糖吸收,GM-CSF释放和ED.
使用SGLT2抑制剂的PWH与不使用这些抑制剂的非糖尿病PWH相比,具有较低的血GM-CSF水平.

结论:

微生物产品可能会驱动内皮糖摄取和GM-CSF表达,导致PWH的炎症和ED.
SGLT2 抑制剂在向这些途径方面表现有前途,在PWH中具有潜在的治疗益处.

关键词:

细胞生物学细胞生物学免疫学免疫学免疫学分子生物学分子生物学病毒学病毒学

更多相关视频

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Published on: January 5, 2016

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

相关概念视频

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Inflammation

Inflammation

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...