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Updated: May 19, 2026

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采用酒精脱水和高折射率介质的创新样本制备,可以获得整个卵细胞的双通道超分辨率3DSTED图像

Michaela Frolikova1, Michaela Blazikova2, Martin Capek2,3

  • 1Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova, Vestec, Czech Republic.

Journal of microscopy
|October 11, 2024
PubMed
概括

使用酒精脱水和高折射率安装的优化样品制备使得大,脆弱的卵子细胞的超分辨率显微镜成为可能. 这一突破克服了样品厚度的限制,可以对生殖生物学结构进行详细的成像.

关键词:
这是一个3D STED.酒精脱水 脱水 酒精脱水高折射率 中等 高折射率 中等大型生物物体是大型生物物体.卵子细胞 卵子细胞样品的准备 样品的准备超级分辨率的超级分辨率

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.