相关实验视频

Updated: Jun 10, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

揭示隐藏的RBP-RNA相互作用与基于RNA修饰酶的策略

Hua Jin^1,2, Chong Li¹, Yunxiao Jia¹

¹Laboratory of Genetics and Disorders, Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing, People's Republic of China.

Wiley interdisciplinary reviews. RNA

|October 11, 2024

概括

本综述探讨了与RNA结合蛋白 (RBPs) 融合的RNA修饰酶 (RMEs),以检测RBP-RNA相互作用. 它涵盖了RME家族,查系统,并比较了基于RME和基于交叉链接和免疫沉 (CLIP) 的分析策略.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

RNA结合蛋白 (RBPs) 是基因表达的关键调节者,影响发育,新陈代谢和疾病.
了解RBP-RNA相互作用对于生物研究和疾病机制至关重要.
最近的进展侧重于将RNA修饰酶 (RME) 与RBPs结合用于相互作用检测的方法.

研究的目的:

审查RNA修饰酶 (ADAR,TENT,AID/APOBEC) 和它们在RBP-RNA相互作用中的作用.
讨论RME活动查,工程突变及其应用.
提供基于RME和基于CLIP的RBP目标分析策略的比较概述.

主要方法:

关于RME家族的文献综述 (ADAR,TENT,AID/APOBEC). 这是一个很好的例子.
分析RME的生物功能,生化活动和基质特异性.
基于RME (例如,TRIBE,STAMP) 和基于CLIP的方法的系统比较,用于RBP目标配置.

主要成果:

详细检查ADAR,TENT和AID/APOBEC蛋白家族,包括它们的域和合作伙伴.
讨论针对RME活动和工程RME变体的选系统.
基于RME和基于CLIP的战略的全面比较,突出了原则,利弊和应用.

结论:

基于RME的方法提供了通过利用酶活性来分析RBP目标的新方法.
将基于RME和基于CLIP的技术进行比较,可以了解选择RBP-RNA相互作用研究的最佳策略.
本次审查巩固了关于RME-RBP融合技术和用于确定RBP目标的传统CLIP方法的当前知识.

关键词:

基因组RNA的修饰是RNA的修饰.标记RNA的标记是RNA的标记.这是一种RNA结合蛋白.盖章盖章是指一个盖章.部落部落部落部落

更多相关视频

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

相关概念视频

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...