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Updated: May 5, 2026

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使用过氧化酶APEX2生物标记方法识别哺乳动物细胞中RNA结合蛋白的内源相互作用者的协议

Ryota Uozumi1, Kohji Mori1, Shoshin Akamine1

  • 1Department of Psychiatry, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.

STAR protocols
|October 11, 2024
PubMed
概括

本研究详细介绍了一种方法,用于识别活细胞中的RNA结合蛋白 (RBP) 相互作用,使用工程化阿斯科巴特过氧化酶2 (APEX2). 该协议使近距离标记和质谱分析能够用于RBP互动原子映射.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学蛋白质的表达和净化过程蛋白质组学是指蛋白质组学.

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