从Chenpi加工残留物中同时回收flavonone和polymethoxyflavonoids,基于它们对降低糖脂活性的互补作用:创新的酵母和水性双相系统的组合使用
Hanliang Li1, Lianzhu Lin1, Mouming Zhao1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technology, Research Center, Guangzhou 510641, China.
Food chemistry
|October 11, 2024
概括
这项研究开发了一种新的生物物理方法,可以从陈皮废物中回收有价值的黄类化合物. 该过程有效地丰富了特定的黄类化合物,具有降糖脂的特性,提供了一种可持续的方法.
科学领域:
- 自然产品化学 自然产品化学
- 生物技术是生物技术.
- 药理学 药理学是指药理学的学科.
背景情况:
- 陈皮加工残留物是精油提取的副产品.
- 黄类药物,特别是黄类药物和聚甲基黄类药物,具有显著的降糖脂活性.
- 需要有效的提取和丰富方法来利用这些有价值的化合物.
研究的目的:
- 开发一种可回收的生物物理方法,用于从陈皮加工残留物回收黄.
- 为了同时丰富flavonone和polymethoxyflavonoids,具有降糖脂活性.
- 为了优化使用酵母发酵和水性双相系统 (ATPS) 提取的净化过程.
主要方法:
- 乙醇反流提取以获得原始的Chenpi黄胺.
- 酵母发酵以去除可减少的糖.
- 使用 (NH4) 2SO4 和乙醇去除水溶性杂质的水性双相系统 (ATPS) 提取.
- 对黄素纯度和成分的分析.
- 通过消化酶抑制,分子对接和网络药理学来评估降糖脂活性.
主要成果:
- 酵母 (2% w/v,CN36) 和ATPS (22% (w/w) (NH4) 2SO4和25% (w/w) 乙醇) 的最佳组合实现了60.25%的黄酸纯度.
- 黄酸盐含量,特别是黄酸盐和聚甲基黄酸盐,增强了6.69倍.
- 纯化的黄酸显示出增强的消化酶抑制活性,优于其他测试方法.
- 该方法有效地去除了可降解糖和水溶性杂质.
结论:
- 成功开发了一种新且可回收的生物物理方法,用于从陈皮加工残留物同时丰富flavonone和polymethoxyflavonoids.
- 这种方法提供了一种可持续和有效的方法,通过回收生物活性化合物来回收农业废物.
- 丰富的黄酸显示出有前途的降甘油脂潜力,得到了体外和体内分析的支持.
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