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人类胚胎干细胞衍生的心血管原生细胞通过激活PI3K/Akt通路来刺激心肌细胞循环活动

Zhongyan Chen¹, Xiujian Yu², Minxia Ke²

¹CAS Key Laboratory of Tissue Microenvironment and Tumor, Laboratory of Molecular Cardiology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences (CAS), CAS, Shanghai 200031, PR China; Zhongshan School of Medicine, Sun Yat-Sen University, Guangdong 510080, PR China.

Journal of molecular and cellular cardiology

|October 11, 2024

概括

人类心血管原生细胞 (hCVPCs) 可以使成人心脏细胞再生. 在心脏病发作的心脏中植入hCVPCs促进宿主心肌细胞脱差和细胞循环重新进入,帮助心脏修复.

科学领域:

心血管生物学心血管生物学
再生医学是一种再生医学.
干细胞疗法干细胞疗法

背景情况:

促进内源性心肌细胞增殖对于在心脏病发作后修复心脏损伤至关重要.
人类多能干细胞衍生的心血管原生细胞 (hCVPCs) 显示出治疗心脏病发作的潜力.
对宿主心肌细胞增殖的hCVPCs的影响仍然在很大程度上是未知的.

研究的目的:

调查hCVPC植入物对心脏病发作的宿主心肌细胞增殖的影响.
阐明hCVPC介导的心肌细胞再生的潜在机制.
探索早期发育阶段细胞对于成年心肌细胞再生的潜力.

主要方法:

植入hCVPCs到老鼠心脏病发作的心脏.
在体外实验中使用hCVPC受条件介质进行实验.
对心肌细胞脱差和细胞循环重新进入的分析.
对PI3K/Akt信号通路的研究.

主要成果:

在心脏梗塞的小鼠心脏中,hCVPC植入诱导了宿主心肌细胞的脱差和细胞周期重新进入.
来自hCVPCs的条件介质在体外复制了这些效应.
确定PI3K/Akt信号通路是hCVPC诱导心肌细胞循环重新进入的媒介.

结论:

hCVPCs在触发心肌细胞脱差和细胞周期激活方面具有新的功能.
hCVPC疗法代表了一种有前途的策略,用于使成人心肌细胞复苏和修复心脏梗塞.
利用早期发育阶段的细胞为心脏再生提供了一个新的途径.

关键词:

心肌细胞脱差分化的过程.心肌细胞的增殖和扩散心血管前代细胞是什么?心肌梗塞的心脏病发作通过PI3K/Akt路径.

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相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...