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ICESp1109,一种新的杂交综合性结合性元素,来自于2003年至2017年期间在波兰收集的抗化物耐药性Streptococcus pyogenes M77血清型

Jan Gawor¹, Karolina Żuchniewicz¹, Matthew Ojeda Saavedra²

¹Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

The Journal of infectious diseases

|October 11, 2024

概括

一种携带 erm(TR) 基因的新型杂交移动遗传元件ICESp1109在波兰的抗宏化物耐药性Streptococcus pyogenes (A组链球菌[GAS]) M77/ST63菌株中被发现. 这一因素在2008年至2017年期间促进了耐药GAS的克隆传播.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

Streptococcus pyogenes (A组链球菌[GAS]) 的抗生素耐药性是一个日益严重的公共卫生问题.
移动遗传元素 (MGE) 在抗生素耐药性基因的传播中起着至关重要的作用.
GAS的M77血清型与侵入性疾病和抗生素耐药性有关.

研究的目的:

在临床分离的Streptococcus pyogenes (A组链球菌[GAS]) M77血清型中研究抗生素耐药性决定因素和相关MGE.
确定这些分离物体中宏类耐药性的遗传背景和演变.
识别有助于GAS抗生素耐药性的新型MGEs.

主要方法:

在波兰 (2003-2017) 收集的136个M77 GAS分离物的全基因组测序 (短读和长读).
生物信息分析用于识别抗生素耐药性基因和MGE.
遗传学分析,以追踪耐药菌株的传播.

主要成果:

所有136个M77 GAS分离物都携带了tet(O) 基因,属于ST63.3序列类型.
在76.5%的隔离物中检测到赋予红色素耐药性的 erm(TR) 基因.
一种新的混合整合性结合元件,ICESp1109,含有 erm(TR),已被确定,主要在2008年后分离的菌株中发现.

结论:

这项研究在抗宏化物耐药的Streptococcus pyogenes中发现了一种新型混合综合性结合元件 (ICESp1109).
遗传学分析显示,2008年至2017年期间,携带ICESp1109的M77/ST63菌株的克隆传播.
ICESp1109代表了格拉姆阳性细菌中重要的新型移动遗传元素,有助于抗生素耐药性的传播.

关键词:

链球菌 pyogenes 的情况.M77/ST63 的时间.基因组测序是指对基因组进行测序.整合性结合元素的整合性结合元素.宏类耐药性宏类耐药性宏类耐药性

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